PRS-00458hu Human C-Reactive Protein,CRP ELISA Kit

Purpose

This kit allows for the determination of CRP concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human CRP level in the sample，use Purified Human CRP antibody to coat microtiter plate wells, make solid-phase antibody, then add CRP to wells, Combined CRP antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit