PRS-02279hu Human Phosphatase And Tensin Homolog (PTEN) ELISA Kit

Product Name: Human Phosphatase And Tensin Homolog (PTEN) ELISA Kit  

Purpose

This kit allows for the determination of PTEN concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human PTEN level in the sample，use Purified Human PTEN antibody to coat microtiter plate wells, make solid-phase antibody, then add PTEN to wells, Combined PTEN antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PTEN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit