Product display

PRS-01414hu Human Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

PRS-01414hu Catalogue No.
Reactivity
Human
Range
200 ng/L-4000 ng/L
Sensitivity
5ng/L
Catalogue NoSpecificationExw Reference Price( USD)Qty Avail
PRS-01414hu48T$268In store
PRS-01414hu96T$368In store



Human Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit


Purpose

This kit allows for the determination of sCD40L concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human sCD40L level in the sampleuse Purified Human sCD40L antibody to coat microtiter plate wells, make solid-phase antibody, then add sCD40L to wells, Combined sCD40L  antibody which With HRP labeledbecome antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of sCD40L  in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1


Closure plate membrane

2

2


Sealed bags

1

1


Microelisa stripplate

1

1

2-8℃

Standard5400 ng/L

0.5ml×1 bottle

0.5ml×1 bottle

2-8℃

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8℃

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8℃

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8℃

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8℃

wash solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8℃


PRECISION

 

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.

 

CV(%) = SD/mean X 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

 

Storage and validity

1Storage:  2-8℃.

2validity: 12 months.