6-Carboxyfluorescein (6-FAM) cas:3301-79-9

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6-Carboxyfluorescein (6-FAM) cas:3301-79-9

CAS: 3301-79-9
Cat NO: C804668
Product Type: Fluorescent Reagent
Product Spec: 95%
MF: C21H12O7
MW: 376.32

English Name:6-Carboxyfluorescein
Molecular Formula:C21H12O7
Molecular Weight: 376.32
CAS: 3301-79-9
Appearance:yellow powder
Packaging:Amber glass screw cap vial (approximately 10 ml).
Absorption Spectrum:Stated
Fluorescence Spectrum:Stated
Identity:Correct wavelengths of absorbance and fluorescence peaks
Purity:99% or better by HPLC
Solubility:Soluble in DMSO or DMF
Storage Conditions:4-8°C
6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495nm and an emission wavelength of 517nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. It is used in the sequencing of nucleic acids and in the labelling nucleotides.
Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.
The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading. It can be incorporated into liposomes, and allow for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells.
Popular derivatives for cell tracing purposes are Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and Carboxyfluorescein succinimidyl ester (CFSE).