The kit is for the quantitative level of Ghrelin in the sample, adopt purified Bird Ghrelin to coat microtiter plate, make
solid-phase antibody, then add Ghrelin to wells, Combine Ghrelin antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured
at a wavelength of 450 nm. The concentration of Ghrelin in the samples is then determined by comparing the O.D. of
the samples to the standard curve.