The kit is for the quantitative level of T4 in the sample, adopt purified Bird Thyroxine (T4) to coat microtiter plate,
make solid-phase antibody, then add T4 to wells, Combine T4 antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured
at a wavelength of 450 nm. The concentration of T4 in the samples is then determined by comparing the O.D. of the
samples to the standard curve.