This kit tests the level of samples rat NDVby using a biotinylated double antigen sandwich enzyme-linked
immunosorbent assay (ELISA) . Add IBV IgMinto the enzyme hole which has been previously coated with rat
NDVmonoclonal antigen,then incubate; after incubation, add the biotin-labeled anti-NDVantibodies, then
combine it with streptavidin -HRP to form immune complexes, remove unbound enzyme through incubation
and washing, and then add substrate a, B, produces blue,and convert into the final yellow in the action of acid .
The depth of color has a positive correlation with the Chicken NDVin the sample .