FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.
This kit allows for the determination of T3 concentrations in Chicken serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Chicken T3 level in the sample，use Purified Chicken T3 antibody to coat microtiter plate wells, make solid-phase antibody, then add T3 to wells, Combined T3 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of T3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.