Chicken Tumor necrosis factor α,TNF-α ELISA KIT

From $368.00

Chicken Tumor necrosis factor α,TNF-α ELISA KIT

Cat NO: PRS-90075Ch
Product Type: Elisa kits
Product Spec: 96T/48T
Wavelength: 450mn

FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.

Purpose

This kit allows for the determination of TNF-α concentrations in Chicken serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Chicken TNF-α level in the sample,use Purified Chicken TNF-α antibody to coat microtiter plate wells, make solid-phase antibody, then add TNF-α to wells, Combined TNF-α antibody which With HRP labeled,become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.