Fluorescein isothiocyanate cas:27072-45-3

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Fluorescein isothiocyanate cas:27072-45-3

CAS: 27072-45-3
Cat NO: F809567
Product Type: Fluorescent reagent
Product Spec: 96%, mixture of 5- and 6- isomers
MF: C21H11NO5S
MW: 389.38

English Name:5(6)-fluorescein isothiocyanate

Molecular Formula:C21H11N05S

Molecular Weight: 389.38

CAS: 27072-45-3

Appearance:Orange powder

Packaging:Amber glass screw cap vial

Absorption Spectrum:Stated

Fluorescence Spectrum:Stated

Identity:Correct wavelengths of absorbance and fluorescence peaks

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
A succinimidyl-ester functional group attached to the fluorescein core, creating “NHS-fluorescein”, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles.
FITC has excitation and emission spectrum peak wavelengths of approximately 495nm/519nm. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.