Generic Name：Goat Beta-Endorphin（β-EP） ELISA Kit.
This kit allows for the determination of β-EP concentrations in Goat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Goat β-EP level in the sample，use Purified Goat β-EP antibody to coat microtiter plate wells, make solid-phase antibody, then add β-EP to wells, Combined β-EP antibody which With HRP labeled ,become antibody – antigen – enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of β-EP in the samples is then determined by comparing the O.D. of the samples to the standard curve.