Hector Battifora mesothelial antigen-1 (HBME-1) ELISA Kit

From $400.00

Hector Battifora mesothelial antigen-1 (HBME-1) ELISA Kit

Cat NO: PR-S11205hu
Product Type: elisa kit
Wavelength: 450nm
Assay range: 2ng/L-85 ng/L
Sensitivity: 0.5 ng/L
Standard: 90ng/L

FOR RESEARCH USE ONLY. Not for clinical diagnosis use

For the quantitative determination of Human HBME-1 concentrations.

Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type
serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 2ng/L-85 ng/L
Sensitivity : 0.5 ng/L

Assay plate (12 × 8 coated Microwells) 1
Standard:90ng/L 1×0.5ml
Standard Diluent 1×1.5ml
HRP-Conjugate Reagent 1×6ml
Sample Diluent 1×6ml
Chromogen Solution A 1×6ml
Chromogen Solution B 1×6ml
Stop Solution 1×6ml
Wash Solution 1×20ml×30 fold
User manual 1
Adhesive Strip 2

Product Principle:
The kit is for the quantitative level of HBME-1 in the sample, adopt purified Human HBME-1 antibody to coat microtiter
plate, make solid-phase antibody, then add HBME-1 to wells, Combine HBME-1 antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes
blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is
measured at a wavelength of 450 nm. The concentration of HBME-1 in the samples is then determined by comparing the O.D.
of the samples to the standard curve.