FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Human HBME-1 concentrations.
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 2ng/L-85 ng/L
Sensitivity : 0.5 ng/L
|Assay plate (12 × 8 coated Microwells)||1|
|Chromogen Solution A||1×6ml|
|Chromogen Solution B||1×6ml|
|Wash Solution||1×20ml×30 fold|
The kit is for the quantitative level of HBME-1 in the sample, adopt purified Human HBME-1 antibody to coat microtiter
plate, make solid-phase antibody, then add HBME-1 to wells, Combine HBME-1 antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes
blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is
measured at a wavelength of 450 nm. The concentration of HBME-1 in the samples is then determined by comparing the O.D.
of the samples to the standard curve.