FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Human α-MSH concentrations.
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 0.3ng/ml-12 ng/ml
|Assay plate (12 × 8 coated Microwells)||1|
|Chromogen Solution A||1×6ml|
|Chromogen Solution B||1×6ml|
|Wash Solution||1×20ml×30 fold|
The kit is for the quantitative level of α-MSH in the sample, adopt purified Human α-MSH antibody to coat microtiter plate,
make solid-phase antibody, then add α-MSH to wells, Combine α-MSH antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of α-MSH in the samples is then determined by comparing the O.D. of the samples
to the standard curve.