Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

From $400.00

Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

Cat NO: PRS-10835hu
Product Type: elisa kit
Product Spec: 96T
Wavelength: 450nm
Assay range: 0.3ng/ml-12 ng/ml
Sensitivity: 0.05ng/ml
Standard: 13.5ng/ml

FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Human α-MSH concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type
serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 0.3ng/ml-12 ng/ml
Sensitivity: 0.05ng/ml
Components:

Assay plate (12 × 8 coated Microwells) 1
Standard:13.5ng/ml 1×0.5ml
Standard Diluent 1×1.5ml
HRP-Conjugate Reagent 1×6ml
Sample Diluent 1×6ml
Chromogen Solution A 1×6ml
Chromogen Solution B 1×6ml
Stop Solution 1×6ml
Wash Solution 1×20ml×30 fold
User manual 1
Adhesive Strip 2

Product Principle:
The kit is for the quantitative level of α-MSH in the sample, adopt purified Human α-MSH antibody to coat microtiter plate,
make solid-phase antibody, then add α-MSH to wells, Combine α-MSH antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of α-MSH in the samples is then determined by comparing the O.D. of the samples
to the standard curve.