Human Interferon γ (IFN-γ) ELISA Kit

From $268.00

Human Interferon γ (IFN-γ) ELISA Kit

Cat NO: PRS-00843hu
Product Type: Elisa kits
Product Spec: 96T/48T
Wavelength: 450mn
Assay range: 1.2 pg/ml -80 pg/ml
Standard: 108 pg/ml

Purpose

This kit allows for the determination of IFN-γ concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human IFN-γ level in the sample,use Purified Human IFN-γ antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-γ to wells, Combined  IFN-γ antibody which With HRP labeled,become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.