This kit allows for the determination of IFN-γ concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human IFN-γ level in the sample，use Purified Human IFN-γ antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-γ to wells, Combined IFN-γ antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.