Human Uric Acid(UA) ELISA Kit
FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.
This kit allows for the determination of UA concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human UA level in the sample， use Purified Human UA antibody to coat microtiter plate wells, make solid-phase antibody, then add UA to wells, Combined UA antibody which With HRP labeled ， become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzymecatalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UA in the samples is then determined by comparing the O.D. of the samples to the standard curve.