For the quantitative determination of human VEGF concentrations.
Detection of species: Human
Detection medium: serum, plasma, Urine, tissue samples, cell culture supernates
PRINCIPLE OF TEST
The kit is for the quantitative level of VEGF in the sample, adopt purified Human VEGF to coat microtiter plate, make
solid-phase antibody, then add VEGF to wells, Combine VEGF antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured
at a wavelength of 450 nm. The concentration of VEGF in the samples is then determined by comparing the O.D. of the
samples to the standard curve.