The kit is for the quantitative level of VEGF A in the sample, adopt purified Human VEGF A to coat microtiter plate, make
solid-phase antibody, then add VEGF A to wells, Combine IL6 antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured
at a wavelength of 450 nm. The concentration ofVEGF A in the samples is then determined by comparing the O.D. of the
samples to the standard curve.