Human Vitamin C(VC) ELISA Kit

From $400.00

Human Vitamin C(VC) ELISA Kit


Details:
 Overview

Reactivity:                Human

Methode Type:        Sandwich ELISA Detection

Quantity:                 96 tests

Sample type:        serum, plasma, Urine,tissue homogenates, cell culture supernates

Detection range :     100ng/L-2000 ng/L

Kit Components:

Assay plate (12 × 8 coated Microwells) ……………………..1

Standard: 2700ng/L…………………………………………  1×0.5ml

Standard Diluent……………………………………………  1×1.5ml

HRP-Conjugate Reagent ……………………………………  1×6ml

Sample Diluent………………………………………………  1×6ml

Chromogen Solution A…………………………………………1×6ml

Chromogen Solution B…………………………………………1×6ml

Stop Solution  …………………………………………………1×6ml

Wash Solution…………………………………………………1×20ml×30 fold

User manual……………………………………………………1

Adhesive Strip……………………………………………………2

Product Principle:

The kit is for the quantitative level of VC in the sample, adopt purified Human VC antibody to coat microtiter plate, make solid-phase antibody, then add VC to wells, Combine VC antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of VC in the samples is then determined by comparing the O.D. of the samples to the standard curve.