Human Vitamin D (VD) ELISA Kit

Price $368.00

Human Vitamin D (VD) ELISA Kit

Cat NO: PRS-00008hu
Product Type: elisa kit
Product Spec: 96T
Wavelength: 450nm
Assay range: 30 nmol/L-1200nmol/L
Standard: 2400 nmol/L

For the quantitative determination of Human VD concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type
serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 30 nmol/L-1200nmol/L

Assay plate (12 × 8 coated Microwells) 1
Standard:2400 nmol/L 1×0.5ml
Standard Diluent 1×1.5ml
HRP-Conjugate Reagent 1×6ml
Sample Diluent 1×6ml
Chromogen Solution A 1×6ml
Chromogen Solution B 1×6ml
Stop Solution 1×6ml
Wash Solution 1×20ml×30 fold
User manual 1
Adhesive Strip 2

Product Principle:
The kit is for the quantitative level of VD in the sample, adopt purified Human VD antibody to coat microtiter plate, make
solid-phase antibody, then add VD to wells, Combine VD antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of VD in the samples is then determined by comparing the O.D. of the samples to
the standard curve.