For the quantitative determination of Human VD concentrations.
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 30 nmol/L-1200nmol/L
|Assay plate (12 × 8 coated Microwells)||1|
|Chromogen Solution A||1×6ml|
|Chromogen Solution B||1×6ml|
|Wash Solution||1×20ml×30 fold|
The kit is for the quantitative level of VD in the sample, adopt purified Human VD antibody to coat microtiter plate, make
solid-phase antibody, then add VD to wells, Combine VD antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of VD in the samples is then determined by comparing the O.D. of the samples to
the standard curve.