Human Zinc transporter 8(ZNT8)ELISA kit

From $400.00

Human Zinc transporter 8(ZNT8)ELISA kit


Details:
 Overview

Reactivity:                Human

Methode Type:        Sandwich ELISA Detection

Quantity:                 96 tests

Sample type:        serum, plasma, Urine,tissue homogenates, cell culture supernates

Detection range :     7ng/L-200 ng/L

Kit Components:

Assay plate (12 × 8 coated Microwells) ……………………..1

Standard: 400ng/L…………………………………………  1×0.5ml

Standard Diluent……………………………………………  1×1.5ml

HRP-Conjugate Reagent ……………………………………  1×6ml

Sample Diluent………………………………………………  1×6ml

Chromogen Solution A…………………………………………1×6ml

Chromogen Solution B…………………………………………1×6ml

Stop Solution  …………………………………………………1×6ml

Wash Solution…………………………………………………1×20ml×30 fold

User manual……………………………………………………1

Adhesive Strip……………………………………………………2

Product Principle:

The kit is for the quantitative level of ZNT8 in the sample, adopt purified Human ZNT8 antibody to coat microtiter plate, make solid-phase antibody, then add ZNT8 to wells, Combine ZNT8 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of ZNT8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.