Human Zonulin ELISA kit

Price $368.00

Human Zonulin ELISA kit

Cat NO: PRS-00802hu
Product Type: elisa kit
Product Spec: 96T
Wavelength: 450nm
Assay range: 30 pg/ml -1500 pg/ml
Standard: 1800 pg/ml

The kit is for the quantitative level of Zonulin in the sample, adopt purified Human Zonulin to coat microtiter plate,
make solid-phase antibody, then add Zonulin to wells, Combine Zonulin antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate
becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of Zonulin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.