The kit is for the quantitative level of Zonulin in the sample, adopt purified Human Zonulin to coat microtiter plate,
make solid-phase antibody, then add Zonulin to wells, Combine Zonulin antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate
becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of Zonulin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.