The kit is for the quantitative level of CD 40L in the sample, adopt purified Mouse CD 40L to coat microtiter plate,
make solid-phase antibody, then add CD 40L to wells, Combine MCP-1 antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate
becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of CD 40L in the samples is then determined by
comparing the O.D. of the samples to the standard curve.