Alternate names:Protease K, Endopeptidase K, Tritirachium alkaline proteinas
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
|DNAses and RNAses||not detected|
One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).
Prepare recombinant Proteinase K with 50 mM Tris-HCl,2 mM Calcium acetate.The optimum pH is 8.0.
Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.