This kit allows for the determination of TNF α concentrations in Rabbit serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rabbit TNF α level in the sample，use Purified Rabbit TNF α antibody to coat microtiter plate wells, make solid-phase antibody, then add TNF α to wells, Combined TNF α antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF α in the samples is then determined by comparing the O.D. of the samples to the standard curve.