Rat interleukin1β(IL-1β) ELISA Kit

From $400.00

Rat interleukin1β(IL-1β) ELISA Kit

Cat NO: PS-20260ra
Product Type: elisa kit
Product Spec: 96T
Wavelength: 450nm
Assay range: 0.8 ng/L-40 ng/L
Standard: 45 ng/L

FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Rat IL-1β concentrations.
Reactivity: Rat
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type
serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 0.8 ng/L-40 ng/L

Assay plate (12 × 8 coated Microwells) 1
Standard:45 ng/L 1×0.5ml
Standard Diluent 1×1.5ml
HRP-Conjugate Reagent 1×6ml
Sample Diluent 1×6ml
Chromogen Solution A 1×6ml
Chromogen Solution B 1×6ml
Stop Solution 1×6ml
Wash Solution 1×20ml×30 fold
User manual 1
Adhesive Strip 2

Product Principle:
The kit is for the quantitative level of IL-1β in the sample, adopt purified Rat IL-1β antibody to coat microtiter plate, make
solid-phase antibody, then add IL-1β to wells, Combine IL-1β antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of IL-1β in the samples is then determined by comparing the O.D. of the samples to
the standard curve.