The kit is for the quantitative level of SOD in the sample, adopt purified Rat SOD to coat microtiter plate, make
solid-phase antibody, then add SOD to wells, Combine SOD antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured
at a wavelength of 450 nm. The concentration of SOD in the samples is then determined by comparing the O.D. of the
samples to the standard curve.