Recombinant Human Chymotrypsin cas:9004-07-3

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Recombinant Human Chymotrypsin cas:9004-07-3

CAS: 9004-07-3
Cat NO: S10001-1
Product Type: Enzyme
Product Spec: ≥1000 USP units/mg pro.

 Recombinant Human Chymotrypsin
CAS: 9004-07-3
Storage temperature: 2-8℃
Source: Recombinant human chymotrypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant human chymotrypsin is identical to human chymotrypsin

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.


Source Recombinant E. coli
Appearance White or off white powder
Specific activity ≥1000 USP units/mg pro.
SDS-PAGE Single major band
SDS-PAGE (MW) 24.0±2.4 kDa

UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine ethyl ester(ATEE) per min at pH 7.0 at 25 °C.

Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.

Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.

Animal origin free: Recombinant human chymotrypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

Used alone, or with other proteases to interact with digestive proteins, or other special uses such as organic synthesis.

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