Sudan red ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Sudan red in milk, honey and meat.The coupling antigens are coated on the micro-well stripes.
The Sudan red in the sample and the coupling antigens coated on the microwell stripes compete for the anti Sudan red antibodies. After the addition of the enzyme conjugate,the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Sudan red in it.
The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Sudan red concentration in the sample.