The Zearalenone (ZEN) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bind with the primary antibody that is complexed to the toxin coated on the plate wells, last, take coloration with TMB substrate. The samples absorbance value is an inverse relationship with ZEN content. Lastly, compared with the standard curve can be concluded the ZEN residues in sample.