Sample Processing is to collect target protein. The processing shall be done as gently as possible as protein denatures or degrades easily. It is also very important to store processed sample, especially avoiding repeated free-thaw cycles. Processed sample can be sub-packed and sealed. Expiry Time: a week at 4 degree; a month at -20 degree; two months at -80 degree. Just put sample in room until its temperature reaches room temperature before using the sample. It is forbidden to heat the sample to melt the sample.
Collect urine sample in sterile tube and then centrifuge at 2000-3000rpm for 20 minutes. Collect supernatant as last.
Notice: it requires to be centrifuged again if sediment appeared while storing sample.